How to Mix HCG Injections- 5000iu

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Please note: These instructions are for mixing hCG from 5000iu vials from places overseas like Escrow Refills, for use with the following standard sized syringes that are typically sent with hCG:

  • 30 unit syringe = .3 ml/cc
  • 50 units syringe = .5 ml/cc
  • 100 unit syringe = 1 ml/cc

READ THIS!! RECENTLY, those who are purchasing BOTH their hCG AND their mixing kits from Escrow Refills are being sent ODD SIZED SYRINGES from Escrow Refills. I have a full post on this here. These odd sized syringes say 40 units = 1 ml/cc on the barrel. IF you get these syringes for use with hCG, PLEASE do NOT mix in the ratio that you see below – it will be too difficult to get your hCG dose because the amount will be so small.  Look at the bottom of this article for full instructions on how to mix your hCG IF you have syringes that say 40 units = 1 ml/cc on them.

How to Mix 5000 iu Vial hCG Injections -

How to mix HCG for HCG Injections: 5000iu

If you have not already purchased hCG, you can check out my HCG Injections page and compare the four companies I currently feel comfortable recommending- all 4 sources are real, unmixed, pharmaceutical hCG – not homeopathic or amino acids, and not premixed.  If you choose to buy through me, I do get a small commission at no extra cost to you.  You totally ROCK if you go through me so thanks!!

Supplies for mixing 1 vial of 5,000IU hCG:

(lasts approx. 3-4 weeks)

  • 1- 5,000iu vial of hCG- will be either a powder or a tablet (buy enough for however many bottles of hcg you will mix for your round).
  • 1- Mixing Syringe (buy enough for however many bottles of hcg you will mix for your round).
  • 1- 10 ml Empty Sterile Vial (buy enough for however many bottles of hcg you will mix for your round).
  • 1- Bacteriostatic Water bottle (this will last for mixing multiple bottles of hcg) Rubbing Alcohol Swabs

Where to Purchase Mixing Supplies

You can buy all the mixing supplies as a kit through either Escrow Refills, along with your hCG (enter my invitation code CID272860 to get on the site, leave the user id and password fields blank), or

The final mix of the hormone will be a solution containing: 5,000IU of hcg + 5ml of bacteriostatic water.  The mixing supplies come all as a kit from escrowrefills.  If you choose to buy the hormone and or mixing/dosing kit from them instructions are here.
If you end up purchasing your hCG from one of the U.S. sources instead of overseas like Escrow Refills, mixing instructions are a little different and you’ll want to go to my injections tutorials page for that.

How to Mix your hCG solution

1. Swab the tops of all bottles with alcohol.
2. Using your mixing syringe, pull out 2mls of bacteriostatic water and insert into your bottle of powdered hcg. Swirl gently until the hcg is completely dissolved.
3. Draw up this same 2ml of bac water that now contains the dissolved hcg with your mixing syringe.
4. Insert this solution into your sterile empty vial.
5. Add 3ml more bacteriostatic water to this same sterile empty vial (that’s not really empty anymore because it contains the 2ml + hCG solution). Done!
     You should now have a vial that contains 5,000IU of hcg + 5ml of bacteriostatic water.

Storing your mixed hCG

Wrap the mixed hCG vial with foil to keep out light. – Store in the refrigerator at all times- remove only to draw out daily injections.
Note: If you accidentally leave the hormone out of the fridge overnight, etc., test the hormone with a pregnancy test to make sure it is still viable- when in doubt, re-mix a new solution with new hCG.


What Dose of hCG Should You Take?

Most people will take anywhere between 125-200IU’s with each dose/injection (1x per day).
Find your dose for no/limited hunger: Most people start with 150IU’s per dose, but 125iu works well for a large majority lately I’ve noticed, and doses of 175iu and 200iu are sometimes needed.  The differences have to do with different brands of hCG, the current level of potency of your mixture, and your own body and its metabolism.
Please see my very detailed post to help you find the right dose of hCG here.

Finding your dose on the syringe

If you have mixed the hormone as I outlined here, the following doses can be found on your insulin syringe in this way: 12.5 units = 125iu 15 units= 150 iu 17.5 units= 175 iu 20 units= 200 iu etc.- see the pattern? The numbers match up with the dose amount, making it easy to adjust your dose as needed without remixing a new batch of hcg. Basically you just move the decimal over one place to the right, and you can then know what that dose equates to.
TIP: If you are having trouble finding a good dose you feel little/no hunger with. Skip a dose entirely one day- if you find yourself more hungry overall, you most likely need to increase your dose. If you find yourself less hungry overall, you most likely need a smaller dose.

How To Do an hCG Injection

(to be done once in the morning daily)
1. Swab the injection site with alcohol (usually in your belly somewhere, at least an inch or so away from your belly button), or alternatively, your upper thigh. Swab the top of the bottle with alcohol as well.
2. Take one injection syringe out and remove the safety cap. Stick the needle into the bottle of hcg and then turn the bottle upside down. Slowly draw out your designated dose (12.5 units= 125iu, 15 units= 150iu, 17.5 units= 175iu, 20 units= 200iu).
3. Remove the needle from the bottle. Pinch the injection site just a little bit, and stick the needle straight in (not at an angle)- you will feel either nothing (if you have a good amount of fat there), or just the slightest twinge (if you don’t have a lot of fat left).
4. Slowly inject the full dose. Then remove the syringe, put the safety cap back on. Dispose of the syringe ideally in a designated container- like a Sharps Container.

Tip 1:

Occasionally, if you stuck the needle in slightly funky, you may get a little bruising, but this is infrequent, and even if it happens, it’s not a big deal.

Tip 2:

Change up the injection sites- switch from one side of the belly to the other each day, etc.

Mixing hCG Injections When You Have the Odd-Sized Syringes

So if you happen to get the weird syringes where it says 40 units = 1 ml/cc, you will mix and dose your injections in a 2:1 ratio instead of a 1:1 ratio.
  • Mix 10 ml’s of bacteriostatic water with 5000iu hCG

Finding hCG Dose for Odd Sized Syringes

If you mix in a 2:1 ratio, the following hCG dosages apply (JUST for the 40 unit = 1 ml/cc syringes):

  • 125ius hCG dose = 10 units
  • 150 ius dose = 12 units
  • 175 ius dose = 14 units
  • 200 ius dose = 16 units
  • 225 ius dose = 18 units
  • 250 ius dose = 20 units


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  • Michelle Olson

    Hey there. I received my new orders from Escrow (thru your discount code – THANKS!)
    & have a question… as I’ve always purchased premixed & thru my USA doctor. I have 3 boxes of Pregnyl 5000 iu. And for some reason they sent me 3 fairly large bottles o bacteria static waters – I thought I ordered 1.
    I aslo have plenty syringes & alcohol swabs.
    In each box with the Pregnyl 5000, there are 2 vials – one is 1ml sodium chloride. Do I not use this first?… to mix the powdered hCG before adding the bacteriostatic waters?

    • Rayzel Lam

      Hi Michelle- So sorry I’m just seeing this comment- you can actually just toss the sodium chloride vial- it’s not enough for mixing the hCG for the diet properly.

  • llfb

    Hi HCG CHICA…I love you videos! just started my first round of is VLCD9 for me…lost 8.6 pounds so far! :) , I have a question…can we inject anywhere in the body where there is fat? is that beneficial?
    I was told to always inject in the belly, in line with belly button.

  • BO

    How do I store my extra Bac water?

  • Mariane Van Durden

    I mixed 2:1 ratio 5000iu hucog 10 bac water. Then I saw your video. Ugh! What is my dose for a 4o day round? I don’t want to throw it away??? 12/30/14. Day 1 loading

    • HCGChica

      Oh that’s okay @marianevandurden:disqus – it’s okay to mix that way as well – this is what the dosages will work out to with the 2:1 ratio:
      25 units – 125 ius
      30 units – 150 ius
      35 units – 175 ius
      40 units – 200 ius

      What type of syringes did you get? What is the total amount the daily dosing syringes hold – it should say at the top. like 3/10 of an ml, or 1 ml, etc.

      • Mariane Van Durden

        Thank you so much for the quick trsponse! Feels food to have you available. The syringe is 1ml/cc 28 gauge 1/2″ easy touch

        I have watched your video since I started back in 2011. I did 2 rounds kept the weight off till August 2013 when I started gaining , was depressed, no energy it was really bad. Finally I found a doc who listened and Was diagnosed with Hashimoto. Been on Westhroid since October 2014 and feeling my old self. I now would like to release the 30 lbs !

        • Mariane Van Durden

          My first three days losses are low .4 daily I am
          Taking westhroid natural thyroid med is this causing the low loss?

          • HCGChica

            Hi @marianevandurden:disqus there are a variety of factors that can cause the weight loss we have. Non optimized thyroid is one of them certainly. But I’ve also discovered my weight loss pattern just varied from round to round too.

  • Marianna Keeth

    I just got my order from Escrow – and my 2 mixing vials are empty – is it supposed to look like that? I ordered 2 of each vial and they look empty – everything else came. Is this normal?

    • HCGChica

      Hey @mariannakeeth:disqus – yes the mixing vials should be empty. Usually they send the mixing kit separately from the hCG itself- the hCG itself will probably come in a little box or something. does that make sense? The mixing vials are what you will put both the bac water and the hCG in.

  • Debra Thomas

    My doctor gave me an instruction sheet saying to add 5,000 ml of the bacteriostatic water to my hcg vial to reconstitute. However, the box has Instructions that say add 10,000 ml to reconstitute! Of course it’s Saturday so I can’t call to confirm.
    The bottle reads “Lyophilized Chorionic Gonadotrophin 10,000 Units/Hydroxocobalamin 5,000 mcg for injections”
    Any suggestions?
    And by the way, a million thanks for your blog!!

  • Debra Thomas

    Hi Rayzel, I hope you can help.
    I received my hcg through my doctor. She instructed me to add 5,000 ml of the bacteriostatic water to reconstitute my hcg. However, the pharmacy instructions on the box direct me to add 10,000 ml.
    The hcg bottle reads “Lyophilized Chorionic Gonadotrophin 10,000 Units/Hydroxocobalmin 5,000 mcg for Injection”
    Any suggestion?
    Thank you for you blog! Wouldn’t be taking this step without it!

    • HCGChica

      Hey @disqus_M5NCxxqhtG:disqus Good questions!
      I prefer the 1:1 ratio that your doctor recommends as well. You can
      technically do it either way. It’s just if you do 2:1 (10ml to 5,000iu)
      then the dosing on the syringe is different. All the instructions on my
      site are for mixing in a 1:1 ratio 5ml to 5000iu hCG.

      Like I
      said, it really doesn’t matter which ratio you do- if you do a more
      diluted ratio of 2:1, you just have to inject more liquid to get the
      same hcG dose that’s all.

      I hope that helps!

  • Lucinda Arizmendi

    Hi my doctor gave me HCG injections already in the syring. Is that bad? He also only put 10units, shouldn’t it be more? Can I redo the syringes?

    • HCGChica

      Hi @Lucinda Arizmendi the problem is that if we don’t know what ratio the hCG was mixed in (how much bac water to how much hCG in the original mixture) then we don’t know what actual dose of hCG is in that 10 units (ie how many iu’s of hCG). So I don’t know the answer there unless we can find out how it was mixed. It’s not necessarily bad to have pre-filled syringes, it’s just that I favor not doing it because it allows a person to easily change their dose of hCG which is often necessary at first to find a dose that leads to least hunger, since this dose is very individual and can even change during the round. I hope that helps a little! – Rayzel

  • Ilissa

    Thanks for the video! How long will the HCG stay for after I mix it? (ie: Would it make sense to mix it today if I am not starting until the weekend?)