Usually the potency of hCG will last between 3-4 weeks. You would notice if the potency had dropped too far to do what it should as your hunger levels would increase.
Since the original protocol had patients doing the hCG Diet in either 3 or 6 week rounds, it seems to work well to use one vial for 3-ish weeks, or remix a 2nd vial of hCG for the following 3 weeks to finish out a longer 6 week round.
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